The pre-steady state reaction of ferrocytochrome c with the cytochrome c-cytochrome aa3 complex
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چکیده
منابع مشابه
Reduction and activity of cytochrome c in the cytochrome c-cytochrome aa3 complex.
Uncharged reductants, such as NNN'N'-tetramethyl-p-phenylenediamine and diaminodurene, reduce cytochrome c at both high and low ionic strength, unlike ascorbate, which is effective only at low ionic strength. The 'tightly bound' cytochrome c-cytochrome c oxidase complex, with 1 equiv. of cytochrome c per cytochrome aa3, can be prepared by simple mixing of the two component species. Its properti...
متن کاملThe cytochrome c peroxidase-catalyzed oxidation of ferrocytochrome c by hydrogen peroxide. Steady state kinetic mechanism.
Initial velocities for the cytochrome c peroxidase-catalyzed oxidation of ferrocytochrome c by hydrogen peroxide have been measured as functions of both the ferrocytochrome c (0.27-104 microM) and hydrogen peroxide (0.25-200 microM) concentrations at 25 degrees C, 0.01 M ionic strength, and pH 7 in a cacodylate/KNO3 buffer system Eadie-Hofstee plots of the initial velocity as a function of ferr...
متن کاملKinetic studies on the reaction between cytochrome c oxidase and ferrocytochrome c.
In stopped-flow experiments in which oxidized cytochrome c oxidase was mixed with ferrocytochrome c in the presence of a range of oxygen concentrations and in the absence and presence of cyanide, a fast phase, reflecting a rapid approach to an equilibrium, was observed. Within this phase, one or two molecules of ferrocytochrome were oxidized per haem group of cytochrome a, depending on the conc...
متن کاملOxidation of ferrocytochrome c by mitochondrial cytochrome c oxidase.
Attempts to rationalize the kinetics of cytochrome c oxidation catalyzed by solubilized mitochondrial cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) have been based on assumptions of productive complex formation (Michaelis-Menten approach). However, the range of substrate concentrations used has not, in general, been sufficient to establish a general rate equation. D...
متن کاملMeasurements of cytochrome c-cytochrome aa3 complex formation by aqueous two-phase partition [proceedings].
Birge, S. J. & Haddad, J. G. (1975) J. Clin. Invest. 56, 1100-1107 Curry, 0. B., Basten, J. F., Francis, M. J . 0. & Smith, R. (1974) Nature (London) 249,83-84 Edwards, R. H. T. (1978) Clin. Sci. Mol. Med. 54,463-470 Haussler, M. R. & McCain, T. A. (1977) N. Engl. J. Med. 297,974-983 Haussler, M. R. & McCain, T. A. (19776) N. Engl. J. Med. 297, 1041-1050 Head, J. F. &Perry, S. V. (1974) Biochen...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 1980
ISSN: 0005-2728
DOI: 10.1016/0005-2728(80)90151-6